Redox variants of NO (NO and HNO) elicit vasorelaxation of resistance arteries via distinct mechanisms

Favaloro JL, Kemp-Harper BK. Redox variants of NO (NO and HNO) elicit vasorelaxation of resistance arteries via distinct mechanisms. Am J Physiol Heart Circ Physiol 296: H1274–H1280, 2009; doi:10.1152/ajpheart.00008.2009.—The free radical form of nitric oxide (NO ) is a well-known mediator of vascular tone. What is not so well recognized is that NO exists in several different redox forms. There is considerable evidence that NO and its one-electron reduction product, nitroxyl (HNO), have pharmacologically distinct actions that extend into the regulation of the vasculature. The aim of this study was to compare the vasorelaxation mechanisms of HNO and NO , including an examination of the ability of these redox variants to hyperpolarize and repolarize vascular smooth muscle cells from rat mesenteric arteries. The HNO donor Angeli’s salt (0.1 nM–10 M) caused a concentration-dependent hyperpolarization of vessels at resting tone and a simultaneous, concentration-dependent vasorelaxation and repolarization of vessels precontracted and depolarized with methoxamine. Both vasorelaxation and repolarization responses to Angeli’s salt were significantly attenuated by both the HNO scavenger L-cysteine (3 mM) and the voltage-dependent K (Kv) channel inhibitor 4-aminopyridine (4-AP; 1 mM) and virtually abolished by the soluble guanylate cyclase (sGC) inhibitor 1H-[1,2,4]oxadiazolo[4,3a]quinoxalin-1-one (ODQ; 10 M) or 30 mM K . In contrast, NO (0.01–1 M) repolarized arteries to a lesser extent than HNO, and these responses were resistant to inhibition by ODQ (10 M) and 4-AP (1 mM). Blockade of Kv channels (1 mM 4-AP) also significantly inhibited the repolarization response to YC-1 (0.1–10 M), confirming a role for sGC/cGMP in the activation of Kv channels in this preparation. We conclude that HNO causes vasorelaxation via a cGMP-dependent activation of Kv channels and that there are different profiles of vasorelaxant activity for the redox siblings HNO and NO .